By G. L. Nicolson (auth.), James K. Koehler Ph. D. (eds.)
The use of the time period "advanced" within the name of this booklet is slightly ar bitrary and intensely a lot relative with admire to time. Many strategies that have been thought of on the "cutting facet" of ultrastructural method quite a few years in the past are actually rou tin ely utilized in a variety of laboratories. possible cite freeze-fracture, cryothin sectioning, or certainly many of the box of experiment ning electron microscopy as concrete examples. hence using the time period "ad vanced recommendations" has to be interpreted in regards to the current cutting-edge, and turns out to be useful merely in informing the aptitude reader that this quantity isn't a primer for use as an preliminary creation into uncomplicated organic elec tron microscopy. many glorious volumes have crammed that area of interest long ago few years, and it isn't meant that this modest booklet be an entire com pendium of the sector. moreover, any restricted number of papers on advanc ed options inevitably displays the personal tastes and arbitrary whims of the editor, thereby apart from many both vital systems which the an expert reader will effortlessly determine. the 1st quantity of this sequence seemed nearly 5 years in the past and illustrated ideas that have been suggestion to symbolize complex and but ba sically morphological equipment for gaining elevated ultrastructural informa tion from organic specimens. the current quantity, nonetheless, stresses thoughts which supply particular physicochemical info at the speci mens as well as the structural information.
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Additional info for Advanced Techniques in Biological Electron Microscopy II: Specific Ultrastructural Probes
J. BioI. Chem. 193,265 - 275 (1951) Majerus, P. , Brodie, G. : The binding of phytohemagglutinins to human platelet plasma membranes. J. BioI. Chem. : Srudies in hemagglutinins of leguminosae seeds. Ann. Med. Exp. Fenn. 35 (suppl. 11), 1-133 (1957) Marchalonis, J. , Edelman, G. : Isolation and characterization of a hemagglutinin from Limulus polyphemus. J. Mol. BioI. 32,453 - 465 (1968) Marchesi, V. : Purification of carbohydrate binding proteins: wheat germ (Triticum vulgaris) agglutinins. Meth.
Biochim. Biophys. , Kostif,]. : Studies on phytohemagglutinins. IX. Metal content and activiry of the hemagglutinin from the lentil (Lens esculenta moench). Biochim. Biophys. : Polysaccharides of Baker's yeast. IV. ]. Chern. Soc. , Nicolson, G. : Quantitative interaction of Ricinus communis agglutinin and concanavalin A with influenza and vesicular stomatitis viruses and virus-infected normal and polyoma-transformed cells. : Membrane splitting in freeze-etching: Covalently bound ferritin as a membrane marker.
HUET et al. lines. If SV 40transformed hamster cells are labeled with native or acetylated Con A at 0° C, they show a continous labeling pattern (Fig. 7 a, b). Con A labeling at - ... " ~ • • ...... ~ ..... C. -----. C. 0 15 30 . -... • II 60 N ..... It ... • .... iI ..... T Fig. 6. Schematic 'glycograms' representing the different rates of redistribution and internalization of the cells labeled and shown in Figure 5. N: Normal secondary hamster embryo cells. T: SV-40 transformed hamster cells.
Advanced Techniques in Biological Electron Microscopy II: Specific Ultrastructural Probes by G. L. Nicolson (auth.), James K. Koehler Ph. D. (eds.)