Read e-book online Amyloid precursor protein : a practical approach PDF

By Weiming Xia, Huaxi Xu

ISBN-10: 0849322456

ISBN-13: 9780849322457

Within the look for a good remedy for Alzheimer's disorder, APP is a different version protein that illustrates the wide variety of simple and complex characterization suggestions to be had.

Exploring various organic thoughts to elucidate the constitution and serve as of this transmembrane protein, this article offers every one strategy with exact, step by step protocols to accomplish reproducible effects and supply a framework for learning different membrane proteins.

Show description

Read Online or Download Amyloid precursor protein : a practical approach PDF

Best basic science books

Download e-book for iPad: Engineering the Human Germline: An Exploration of the by Gregory Stock

This e-book explores the numerous clients, demanding situations and moral questions that encompass the engineering of our reproductive cells. it's an obtainable, three-part exam, relocating from targeted, sensible checks of the promise and difficulties for this advancing expertise to a bit of brief essays at the implications of our technological skill.

Michael Wilson's Bacterial disease mechanisms : an introduction to cellular PDF

This introductory textbook explores bacterial illness mechanisms and bacteria-host interactions. The primary premise is that micro organism have advanced by way of manipulating common host cellphone capabilities and overcoming host safeguard structures to make sure their survival. in addition to supplying a brand new standpoint at the classical bacterial virulence mechanisms, this booklet outlines the hot molecular options constructed to resolve the complexity of bacteria-host interactions.

Cardiovascular Physiology Neural Control Mechanisms. - download pdf or read online

Cardiovascular body structure Neural regulate Mechanisms comprises the court cases of the symposia of the twenty eighth overseas Congress of body structure held in Budapest among thirteen and 19 of July, 1980. geared up into six elements, this booklet starts off with an elucidation of the integrative position of the autonomic anxious process within the law of cardiovascular functionality.

Download e-book for kindle: Tetrahymena thermophila by David J Asai; James D Forney; American Society for Cell

Tetrahymena thermophila is rising as a robust experimental process for useful genetics. The ciliated protozoan deals various merits, no longer the least of that's the facility to dispose of any particular gene of curiosity after which to guage the impact of that mutation at the dwelling cellphone. previous investigations with T.

Additional info for Amyloid precursor protein : a practical approach

Sample text

21. U. et al. The cytoplasmic domain of the LDL receptor-related protein regulates multiple steps in APP processing. EMBO J. 21, 5691, 2002. 22. H. Trafficking of cell surface beta-amyloid precursor protein: retrograde and transcytotic transport in cultured neurons. J. Cell Biol. 129, 431, 1995. 23. A. A. Rab15 mediates an early endocytic event in Chinese hamster ovary cells. J. Biol. Chem. 274, 22303, 1999. fm Page 29 Wednesday, November 3, 2004 2:59 PM 3 Strategies for Crystallizing the N-Terminal Growth Factor Domain of Amyloid Precursor Protein William J.

3 KINETICS OF SECRETION AND ENDOCYTOSIS OF APP This protocol examines the trafficking of APP by pulse/chase analysis. A population of surface APP molecules is initially bound to radiolabeled antibody at 4˚C and is then, after removal of unbound antibody, allowed to transit when rewarmed to 37˚C. At various time points, the supernatant containing the secreted APP is collected and any remaining antibody is removed from the cell surface by acid washes. Finally, the cell pellet is lysed (acid resistant fraction) to obtain the internalized APP fraction and all samples are measured in a gamma counter.

Crystal structure of the N-terminal, growth factor-like domain of Alzheimer amyloid precursor protein. Nature Struct. Biol. 6, 327, 1999. 25. Hilbich, C. et al. Amyloid-like properties of peptides flanking the epitope of amyloid precursor protein-specific monoclonal antibody 22C11. J. Biol. Chem. 268, 26571, 1993. 26. , Crystallization of Biological Macromolecules, Cold Spring Harbor Laboratory Press, New York, 1999. 27. M. et al. Amino-terminal region of the beta-amyloid precursor protein activates mitogen-activated protein kinase.

Download PDF sample

Amyloid precursor protein : a practical approach by Weiming Xia, Huaxi Xu

by Daniel

Rated 4.59 of 5 – based on 21 votes